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ASTM E 2144 Document Information:
Title
Standard Practice for Personal Sampling and Analysis of Endotoxin in Metalworking Fluid Aerosols in Workplace Atmospheres
ASTM International
Publication Date:
Apr 10, 2001
Scope:
This practice covers quantitative methods for the personal
sampling and determination of bacterial endotoxin concentrations in
poly-disperse metalworking fluid aerosols in workplace atmospheres.
Users should have fundamental knowledge of microbiological
techniques and endotoxin testing.
Users of this practice may obtain personal or area exposure data
of endotoxin in metalworking fluid aerosols, either on a short-term
or full-shift basis in workplace atmospheres.
This practice gives an estimate of the endotoxin concentration
of the sampled atmosphere.
This practice seeks to minimize inter laboratory variation but
does not ensure uniformity of results.
It is anticipated that this practice will facilitate inter
laboratory comparisons of airborne endotoxin data from metalworking
fluid atmospheres, particularly metal removal fluid atmospheres, by
providing a basis for endotoxin sampling, extraction, and
analytical methods.
In 1997, the Occupational Safety and Health Administration
(OSHA) empanelled a Standards Advisory Committee to make
recommendations to the Administration regarding measures that the
Administration could take to improve the health of workers exposed
to metalworking fluids. A report to the Assistant Secretary of
Labor for OSHA was submitted in July, 1999. Subcommittee E34.50
believes that the user community would benefit significantly if a
standard method was developed to give the community guidance on a
methodology for the sampling and analysis of personal airborne
endotoxin exposure assessments in facilities using water-miscible
metal removal fluids, based on the LAL assay or other endotoxin
detection technologies as they become available.
This practice does not attempt to set or imply limits for
personal exposure to endotoxin in metalworking fluid aerosols in
workplace environments.
This standard does not purport to address all of the safety
concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and
health practices and determine the applicability of regulatory
limitations prior to use.
Keywords:
- aerosol sampling
- airborne endotoxin
- depyrogenation
- endotoxin
- endotoxin assay
- endotoxin units (EU)
- extraction methods
- Gram-negative bacteria
- Limulus amebocyte lysate (LAL) assay
- metalworking fluid aerosols
- metalworking fluids
- metal removal fluids
- sampling and analysis
- workplace atmospheres
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