ASTM E 1470 Standard Test Method for Characterization of Proteins by Electrophoretic Mobility

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ASTM E 1470 Document Information:

Title
Standard Test Method for Characterization of Proteins by Electrophoretic Mobility

ASTM International

Publication Date:
Mar 15, 1992

Scope:

This test method describes a procedure for determining the electrophoretic mobility of proteins of molecular weight greater than 10 000 Daltons.

This test method uses automatic Electrophoretic Light Scattering (ELS) principles to determine the electrophoretic mobility.

The instrument² simultaneously measures the Doppler shifts of scattered light at four different angles to determine the electrophoretic mobility distribution of protein particles. The mobility is expressed as µm-cm/V-s (micron-centimeter/volt-second).

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

² The sole source of supply of the instrument (Coulter Delsa 440, trademark) known to the committee at this time is Coulter Corporation, 601 W. Coulter Way, Hialeah, FL 33010. If you are aware of alternative suppliers, please provide this information to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of the responsible technical committee,¹ which you may attend.

¹ This test method is under the jurisdiction of ASTM Committee E48 on Biotechnology and is the direct responsibility of Subcommittee E48.03 on Unit Processes and Validation.

Keywords:

molecular weight
electrophoretic mobility
proteins

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