ASTM E 2250 Standard Method for Determination of Endotoxin Concentration in Water Miscible Metal Working Fluids
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ASTM E 2250 Document Information:
Title
Standard Method for Determination of Endotoxin Concentration in Water Miscible Metal Working Fluids
ASTM International
Publication Date:
Oct 10, 2002
Scope:
This method covers quantitative methods for the sampling and
determination of Gram-negative
bacterial endotoxin concentrations in water miscible metalworking
fluids (MWF).
Users of this method should be familiar with the handling of MWF.
This method gives an estimate of the endotoxin concentration of the
sampled MWF.
Used on site, this method gives an indication of changes in
Gram-negative bacterial contamination
in the MWF.
This method does not replace Practice E 2144.
This method seeks to minimize inter-laboratory variation but does not
ensure uniformity of results.
This method is not intended to relate endotoxin concentration in MWF
to health effects of inhaled
endotoxin.
This standard does not purport to address all of the safety concerns,
if any, associated with its
use. It is the responsibility of the user of this standard to
establish appropriate safety and
health practices and determine the applicability of regulatory
limitations prior to use.
Keywords:
- endotoxin
- endotoxin assay
- endotoxin units (EU)
- fluorescence polarization
- Gram-negative bacteria
- MWF
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