ASTM D 4148 Standard Test Method for Analysis of Phytoplankton in Surface Water by the Sedgwick-Rafter Method
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ASTM D 4148 Document Information:
Title
Standard Test Method for Analysis of Phytoplankton in Surface Water by the Sedgwick-Rafter Method
ASTM International
Publication Date:
Jun 25, 1982
Scope:
This test method covers determining the density and taxonomic
classification of phytoplankton. It
is applicable both to relatively sparse or dense phytoplankton
concentrations, provided the
suspended-sediment concentration is low. The Sedgwick Rafter (S-R)
method requires less costly
apparatus than does the inverted microscope method but gives less
accurate results. The inherent
inaccuracy in the Sedgwick-Rafter method is due to the design of the
counting chamber and cannot be
circumvented by a different choice of optics. For this reason, the S-R
method is limited to the use
of objective lenses having a working distance of approximately 1.6 mm
or more. With 10×
oculars the maximum overall magnification is approximately 250×.
High concentrations of
suspended sediment can obscure the algal cells, and thus cause
interference.
This test method is applicable to both freshwater and marine samples.
This standard does not purport to address all of the safety problems,
if any, associated with its
use. It is the responsibility of the user of this standard to
establish appropriate safety and
health practices and determine the applicability of regulatory
limitations prior to use. For
specific precautionary information see Section 8.
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