ASTM E 1482 Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations
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ASTM E 1482 Document Information:
Title
Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations
ASTM International
Publication Date:
Oct 1, 2004
Scope:
This test method covers the use, in conjunction with evaluations of
the virucidal efficacy, of
disinfectant solutions or pressurized disinfectant spray products
intended for use on inanimate
nonporous environmental surfaces or for other special applications.
The test method may be employed
with all viruses and host systems.
This test method should be performed only by persons trained in
microbiology and virology.
This test method utilizes gel filtration technology. The effectiveness
of the test method is
dependent on the ratio of gel bed volume to sample size and uniformity
in the preparation of
columns and centrifugation conditions. The effectiveness of this test
method is maximized by
investigator practice and experience with gel filtration techniques.
This test method will reduce, but not necessarily eliminate,
disinfectant toxicity while preserving
the titer of input virus.
The values stated in SI units are to be regarded as the standard.
This standard does not purport to address all of the safety concerns,
if any, associated with its
use. It is the responsibility of the user of this standard to
establish appropriate safety and
health practices and determine the applicability of regulatory
limitations prior to use.
Keywords:
- cytotoxicity
- disinfectant
- gel filtration
- neutralization
- tissue culture
- virucidal
- virucidal neutralization method
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