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ASTM E 1022 Document Information:
Title
Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks
ASTM International
Publication Date:
Jan 1, 1994
Scope:
This guide describes procedures for obtaining laboratory data
concerning bioconcentration of a test material added to dilution
water—but not to food—by freshwater and saltwater fishes and
saltwater bivalve mollusks using the flow-through technique. These
procedures also should be useful for conducting bioconcentration
tests with other aquatic species, although modifications might be
necessary.
Other modifications of these procedures might be justified by
special needs or circumstances. Although using appropriate
procedures is more important than following prescribed procedures,
the results of tests conducted using unusual procedures are not
likely to be comparable to those of many other tests. The
comparison of results obtained using modified and unmodified
versions of these procedures might provide useful information
concerning new concepts and procedures for conducting
bioconcentration tests.
These procedures are applicable to all chemicals that can be
measured accurately at the necessary concentrations in water and in
appropriate tissues. Bioconcentration tests are usually conducted
on individual chemicals but can be conducted on mixtures if
appropriate measurements can be made. Some techniques described in
this guide were developed for tests on non-ionizable organic
chemicals (see 11.1.2.1) and might not apply to ionizable or
inorganic chemicals.
Results of bioconcentration tests should usually be reported in
terms of apparent steady-state and projected steadystate
bioconcentration factors (BCFs) and uptake and depuration rate
constants. Results should be reported in terms of whole body for
fishes and in terms of total soft tissue for bivalve mollusks. For
fishes and scallops consumed by humans, some results should also be
reported in terms of the edible portion, especially if ingestion of
the test material by humans is a major concern. For tests on
organic and organometallic chemicals, the percent lipids of the
tissue should be reported.
The values stated in SI units are to be regarded as the
standard. The values given in parentheses are for information
only.
This standard does not purport to address all of the safety
concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and
health practices and determine the applicability of regulatory
limitations prior to use. Specific precautionary statements
are given in Section 7.
Keywords:
- bioaccumulation
- bioconcentration
- bioconcentration factor (BCF)
- bivalve mollusks
- depuration phase
- fishes
- uptake phase
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