ASTM E 1262 Standard Guide for Performance of Chinese Hamster Ovary Cell/Hypoxanthine Guanine Phosphoribosyl Transferase Gene Mutation Assay
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ASTM E 1262 Document Information:
Title
Standard Guide for Performance of Chinese Hamster Ovary Cell/Hypoxanthine Guanine Phosphoribosyl Transferase Gene Mutation Assay
ASTM International
Publication Date:
Jul 29, 1988
Scope:
This guide highlights some of the more relevant biological
concepts as they are currently understood, and summarizes the
critical technical aspects for acceptable bioassay performances as
they currently are perceived and practiced. The Chinese hamster
ovary cell/hypoxanthine guanine phosphoribosyl transferase
(CHO/HGPRT) assay (1)2 has been widely applied to the
toxicological evaluation of industrial and environmental
chemicals.
This guide concentrates on the practical aspects of cell
culture, mutagenesis procedures, data analysis, quality control,
and testing strategy. The suggested approach represents a consensus
of the panel members for the performance of the assay. It is to be
understood, however, that these are merely general guidelines and
are not to be followed without the use of sound scientific
judgement. Users of the assay should evaluate their approach based
on the properties of the substances to be tested and the questions
to be answered.
Deviation from the guidelines based on sound scientific
judgement should by no means invalidate the results obtained.
The values stated in SI units are to be regarded as standard. No
other units of measurement are included in this standard.
This standard does not purport to address all of the safety
concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and
health practices and determine the applicability of regulatory
limitations prior to use.
2 The boldface numbers in parentheses refer to the
list of references at the end of this guide.
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