ASTM E 1532 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome

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ASTM E 1532 Document Information:

Title
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome

ASTM International

Publication Date:
May 10, 2000

Scope:

This practice covers the use of cell cultures and DNA-binding flurorochrome techniques to detect mycoplasma contamination of cell cultures.

This practice does not cover axenic cultivation or identification of mycoplasmas.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords:

DNA stain

indicator cells

mycoplasma

cell culture

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