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ASTM E 1611 Document Information:
Title
Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids
ASTM International
Publication Date:
Apr 10, 2000
Scope:
This guide covers procedures for obtaining laboratory data
concerning the adverse effects of potentially contaminated
sediment, or of a test material added experimentally to
contaminated or uncontaminated sediment, on marine or estuarine
infaunal polychaetes during 10-day or 20 to 28-day exposures. These
procedures are useful for testing the effects of various
geochemical characteristics of sediments on marine and estuarine
polychaetes and could be used to assess sediment toxicity to other
infaunal taxa, although modifications of the procedures appropriate
to the test species might be necessary. Procedures for the 10-day
static test are described for Neanthes arenaceodentata and
Neanthes virens and for the 20 to 28-day staticrenewal
sediment toxicity for N. arenaceodentata.
Modifications of these procedures might be appropriate for other
sediment toxicity test procedures, such as flowthrough or partial
life-cycle tests. The methods outlined in this guide should also be
useful for conducting sediment toxicity tests with other aquatic
taxa, although modifications might be necessary. Other test
organisms might include other species of polychaetes, crustaceans,
and bivalves.
Other modifications of these procedures might be appropriate for
special needs or circumstances. Although using appropriate
procedures is more important than following prescribed procedures,
the results of tests conducted using unusual procedures are not
likely to be comparable to those of many other tests. Comparisons
of the results obtained using modified and unmodified versions of
these procedures might provide useful information concerning new
concepts and procedures for conducting sediment tests with infaunal
organisms.
These procedures are applicable to sediments contaminated with
most chemicals, either individually or in formulations, commercial
products, and known or unknown mixtures. These procedures can be
used with appropriate modifications to conduct sediment toxicity
tests on factors such as temperature, salinity, dissolved oxygen
(DO), and natural sediment characteristics (for example, particle
size distribution, organic carbon content, and total solids). These
procedures can also be used to conduct bioconcentration tests and
in situ tests, and to assess the toxicity of potentially
contaminated field sediments, or of materials such as sewage
sludge, oils, particulate matter, and solutions of toxicants added
to sediments. A median lethal concentration (LC50) or median
sublethal effect concentration (EC50) of toxicants or of highly
contaminated sediment mixed into uncontaminated sediment can be
determined. Materials adhering to sediment particles or dissolved
in interstitial water can be tested.
The results of 10-day toxicity tests with contaminated sediments
can be reported as a LC50 if a series of concentrations is tested
or as a percent mortality relative to a control or reference
sediment. The results of 20 to 28-day toxicity tests with
contaminated sediments can be reported as a LC50 if a series of
concentrations is tested or as a percent mortality or growth
relative to a control or reference sediment.
The values stated in SI units are to be regarded as the
standard.
This standard does not purport to address all of the safety
concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and
health practices and determine the applicability of regulatory
limitations prior to use. Specific hazards statements are
given in Section 8.
*A
Summary of Changes section appears at the end of this
standard.
Keywords:
- acute toxicity tests
- chronic toxicity tests
- estuarine
- environments
- experimental design
- exposure tests
- growth
- LC50 test
- marine environments
- Neanthes
- polychaetes
- reference
- toxicants
- saline water
- saltwater
- sediment
- sediment
- toxicity testing
- static test
- terminology
- toxicity
- toxicology
- 10-day test
- 20 to 28-day test
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